6-Shogaol reduces progression of experimental endometriosis in vivo and in vitro via regulation of VGEF and inhibition of COX-2 and PGE2-mediated inflammatory responses.
10.4196/kjpp.2018.22.6.627
- Author:
Dan WANG
1
;
Yiling JIANG
;
Xiaoxin YANG
;
Qiong WEI
;
Huimin WANG
Author Information
1. Department of Obstetrics and Gynecology, Tongren Hospital of WuHan University (Wuhan Third Hospital), Wuhan 430074, China.
- Publication Type:Original Article
- Keywords:
6-Shogaol;
Angiogenesis;
Endometriosis;
Inflammatory mediators;
NF-κB
- MeSH:
Abdominal Wall;
Atrophy;
Dinoprostone;
Endometriosis*;
Endometrium;
Female;
Gestrinone;
Humans;
In Vitro Techniques*;
Interleukin-6;
Nitric Oxide;
Peritoneum;
Rats, Sprague-Dawley;
Vascular Endothelial Growth Factor A;
Vascular Endothelial Growth Factor Receptor-2
- From:The Korean Journal of Physiology and Pharmacology
2018;22(6):627-636
- CountryRepublic of Korea
- Language:English
-
Abstract:
Endometriosis (EM) is one of the most common gynaecological disorder affecting women in their reproductive age. Mechanisms involved in the pathogenesis of EM remains poorly understood, however inflammatory responses have been reported to be significantly involved. The efficacy of 6-shogaol on proliferation of endometriotic lesions and inflammatory pathways in experimentally-induced EM model was explored in this study. EM was stimulated in Sprague-Dawley rats by implantation of autologous endometrium onto the peritoneum abdominal wall. Separate groups were treated with 6-shogaol (50, 100 or 150 mg/kg b.wt/day) via oral gavage for one month period. Gestrinone (GTN) group received GTN (0.5 mg/kg/day) as positive control. Five weeks after implantation, the spherical volume of ecto-uterine tissues was determined. Treatment with 6-shogaol significantly reduced the implant size. Histological analysis reported atrophy and regression of the lesions. 6-shogaol administration effectively down-regulated NF-κB signaling, VEGF and VEGFR-2 (Flk-1) expression in the endometriotic lesions. Excess production of IL-1β and IL-6 (pro-inflammatory cytokines), PGE2 and nitric oxide (NO) were reduced. Overall, the results of the study reveal the efficacy of 6-shogaol against endometriosis via effectively suppressing proliferation of the lesions and modulating angiogenesis and COX-2/NF-κB-mediated inflammatory cascades.
