The treatment effect of novel hGHRH homodimer to male infertility hamster.
10.4196/kjpp.2018.22.6.637
- Author:
Xu Dong ZHANG
1
;
Xiao Yuan GUO
;
Jing Xuan TANG
;
Lin Na YUE
;
Juan Hui ZHANG
;
Tao LIU
;
Yu Xia DONG
;
Song Shan TANG
Author Information
1. Department of Biochemistry and Molecular Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. songstang@hotmail.com
- Publication Type:Original Article
- Keywords:
Autocrine;
Chines hamster;
Growth hormone releasing hormone;
Homodimer;
Male infertility
- MeSH:
Animals;
Cricetinae*;
Cricetulus;
Cyclophosphamide;
Fertility;
Gonadotropins;
Growth Hormone-Releasing Hormone;
Humans;
In Situ Nick-End Labeling;
Infertility;
Infertility, Male*;
Male;
Male*;
Pregnancy Rate;
Reproduction;
Testis;
Testosterone;
Vacuoles
- From:The Korean Journal of Physiology and Pharmacology
2018;22(6):637-647
- CountryRepublic of Korea
- Language:English
-
Abstract:
Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in reproduction. To study the treatment effect of Grin (a novel hGHRH homodimer), the infertility models of 85 male Chinese hamsters were established by intraperitoneally injecting 20 mg/kg of cyclophosphamide once in a week for 5 weeks and the treatment with Grin or human menopausal gonadotropin (hMG) as positive control was evaluated by performing a 3-week mating experiment. 2–8 mg/kg of Grin and 200 U/kg of hMG showed similar effect and different pathological characteristics. Compared to the single cyclophosphamide group (0%), the pregnancy rates (H-, M-, L-Grin 26.7, 30.8, 31.3%, and hMG 31.3%) showed significant difference, but there was no difference between the hMG and Grin groups. The single cyclophosphamide group presented loose tubules with pathologic vacuoles and significant TUNEL positive cells. Grin induced less weight of body or testis, compactly aligned tubules with little intra-lumens, whereas hMG caused more weight of body or testis, enlarging tubules with annular clearance. Grin presented a dose-dependent manner or cell differentiation-dependentincrease in testicular GHRH receptor, and did not impact the levels of blood and testicular GH, testosterone. Grin promotes fertility by proliferating and differentiating primitive cells through up-regulating testicular GHRH receptor without triggering GH secretion, which might solve the etiology of oligoasthenozoospermia.
