Tussilagone suppressed the production and gene expression of MUC5AC mucin via regulating nuclear factor-kappa B signaling pathway in airway epithelial cells.
10.4196/kjpp.2018.22.6.671
- Author:
Byung Soo CHOI
1
;
Yu Jin KIM
;
Yong Pill YOON
;
Hyun Jae LEE
;
Choong Jae LEE
Author Information
1. Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Korea. LCJ123@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Airway;
Epithelium;
Gene expression;
Mucin;
Tussilagone
- MeSH:
Blotting, Western;
Down-Regulation;
Enzyme-Linked Immunosorbent Assay;
Epidermal Growth Factor;
Epithelial Cells*;
Epithelium;
Gene Expression*;
Mucins*;
NF-kappa B;
Phosphorylation;
Phosphotransferases;
Tussilago
- From:The Korean Journal of Physiology and Pharmacology
2018;22(6):671-677
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, we investigated whether tussilagone, a natural product derived from Tussilago farfara, significantly affects the production and gene expression of airway MUC5AC mucin. Confluent NCI-H292 cells were pretreated with tussilagone for 30 min and then stimulated with EGF (epidermal growth factor) or PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. To elucidate the action mechanism of tussilagone, effect of tussilagone on PMA-induced NF-κB signaling pathway was investigated by western blot analysis. Tussilagone significantly inhibited the production of MUC5AC mucin protein and down-regulated the expression of MUC5AC mucin gene, induced by EGF or PMA. Tussilagone inhibited PMA-induced activation (phosphorylation) of inhibitory kappa B kinase (IKK), and thus phosphorylation and degradation of inhibitory kappa Ba (IκBα). Tussilagone inhibited PMA-induced phosphorylation and nuclear translocation of nuclear factor kappa B (NF-κB) p65. This, in turn, led to the down-regulation of MUC5AC protein production in NCI-H292 cells. These results suggest that tussilagone can regulate the production and gene expression of mucin by acting on airway epithelial cells through regulation of NF-κB signaling pathway.
