Two dinucleotide repeat polymorphisms (AC/TG and GT/CA) in the 5' upstream region of the mouse tryptophan hydroxylase gene.
- Author:
Sung Vin YIM
1
;
Sung Gil CHI
;
Sung Hyun CHUNG
;
Hee Jae LEE
;
Mi Ja KIM
;
Seung Joon PARK
;
Jee Chang JUNG
;
Joo Ho CHUNG
Author Information
1. Department of Pharmacology, College of Medicine, Kyung Hee University, 1 Hoegi-Dong, Tongdaemun-Ku, Seoul, 130-701 South Korea.
- Publication Type:Original Article
- MeSH:
Alleles;
Animals;
Dinucleotide Repeats*;
DNA;
Humans;
Mice*;
Mice, Inbred ICR;
Microsatellite Repeats;
Nucleotides;
Polymerase Chain Reaction;
Raphe Nuclei;
Sequence Analysis, DNA;
Serotonergic Neurons;
Serotonin;
Tandem Repeat Sequences;
Tryptophan Hydroxylase*;
Tryptophan*
- From:The Korean Journal of Physiology and Pharmacology
1999;3(5):501-505
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin biosynthesis, is primarily expressed in serotonergic neurons of the raphe nuclei. Simple tandem repeat polymorphisms, typically one to four nucleotides long, are tandemly repeated several times and often characterized by many alleles. To identify the presence of polymorphic repeats, we sequenced the 5'-upstream region of the mouse TPH gene. For the detection of any allelic variants, polymerase chain reaction, nonisotopic single-strand conformation polymophism, and DNA sequencing analyses of the tandem repeat sequences were performed using genomic DNA extracted from 60 ICR mice. Two dinucleotide repeats, 5'-(AC/TG)22-3' and 5'-(GT/CA)17-3', were identified at approximately -5.7 kb and -3.4 kb upstream from the transcriptional initiation site of the mouse TPH gene, respectively. Minor allelic variants, 5'-(AC/TG)21-3' and 5'-(GT/CA)18-3', were observed in heterozygous pairs from 3 of 60 and 1 of 60 ICR mice, respectively. The identification of these microsatellites in the mouse TPH promoter raises the possibility that identical and/or other polymorphic sequences might exist in the upstream region of the human TPH gene.
