Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats.
10.4196/kjpp.2015.19.1.43
- Author:
Minjae JOO
1
;
Han Sang KIM
;
Tae Hoon KWON
;
Alisha PALIKHE
;
Tin Sandar ZAW
;
Ji Hoon JEONG
;
Uy Dong SOHN
Author Information
1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. udsohn@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Colitis;
Flavonoids;
Inflammation;
Quercetin;
Reactive oxygen species
- MeSH:
Animals;
Colitis*;
Colon;
Esophagus;
Flavonoids*;
Glutathione;
Inflammation;
Inflammatory Bowel Diseases;
Malondialdehyde;
Models, Animal;
Neutrophil Activation;
Nitric Oxide;
Oxidative Stress;
Peroxidase;
Quercetin;
Rats*;
Reactive Oxygen Species;
Stomach;
Tumor Necrosis Factor-alpha;
Ulcer
- From:The Korean Journal of Physiology and Pharmacology
2015;19(1):43-50
- CountryRepublic of Korea
- Language:English
-
Abstract:
It has been shown that the extracts including eupatilin and quercetin-3-beta-D-glucuronopyranoside had mucoprotective effects on the esophagus and stomach through their antioxidant activities. This study was designed to investigate the anti-inflammatory effect of these flavonoid compounds in an animal model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulfonic acid. Experimental colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid. Extracts including eupatilin or quercetin-3-beta-D-glucuronopyranoside were orally administered to animals 48, 24, and 1 h prior to the induction of colitis and then again 24 h later. The animals were sacrificed 48 h after by 2,4,6-trinitrobenzene sulfonic acid treatment and the macroscopic appearance of the colonic lesions was scored in a blinded manner on a scale of 1 to 10. The inflammatory response to colitis induction was assessed by measuring myeloperoxidase activity, nitric oxide production, tumor necrosis factor-alpha expression, total glutathione levels, and malondialdehyde concentrations in the colon. The results indicated that extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside dose-dependently improved the morphology of the lesions induced by 2,4,6-trinitrobenzene sulfonic acid and reduced the ulcer index accordingly. In addition, rats receiving extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside showed significantly decreased levels of mucosal myeloperoxidase activity, nitric oxide production, tumor necrosis factor-alpha expression, and malondialdehyde levels, and increased total glutathione levels. Extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside ameliorated the inflammatory response and colonic injury in acute colitis by decreasing oxidative stress and neutrophil activation. Extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside may inhibit acute colitis.
