Morinda citrifolia Inhibits Both Cytosolic Ca2+-dependent Phospholipase A2 and Secretory Ca2+-dependent Phospholipase A2.
10.4196/kjpp.2010.14.3.163
- Author:
Ho Sun SONG
1
;
Sung Hun PARK
;
Myoung Soo KO
;
Jae Min JEONG
;
Uy Dong SOHN
;
Sang Soo SIM
Author Information
1. Department of Pathophysiology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. simss@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Morinda citrifolia;
Phospholipase A2;
Arachidonic acid
- MeSH:
Arachidonic Acid;
Cytosol;
Hydrolysis;
Methanol;
Morinda;
Phosphatidylcholines;
Phospholipases;
Phospholipases A2
- From:The Korean Journal of Physiology and Pharmacology
2010;14(3):163-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the effects of the methanol extracts of Morinda citrifolia containing numerous anthraquinone and iridoid on phospholipase A2 (PLA2) isozyme. PLA2 activity was measured using various PLA2 substrates, including 10-pyrene phosphatidylcholine, 1-palmitoyl-2-[14C]arachidonyl phosphatidylcholine ([14C]AA-PC), and [3H]arachidonic acid (AA). The methanol extracts suppressed melittin-induced [3H]AA release in a concentration-dependent manner in RAW 264.7 cells, and inhibited cPLA2/sPLA2-induced hydrolysis of [14C]AA-PC in a concentration- and time-dependent manner. A Dixon plot showed that the inhibition by methanol extracts on cPLA2 and sPLA2 appeared to be competitive with inhibition constants (Ki ) of 3.7microgram/ml and 12.6microgram/ml, respectively. These data suggest that methanol extracts of Morinda citrifolia inhibits both Ca2+-dependent PLA2 such as, cPLA2 and sPLA2. Therefore, Morinda citrifolia may possess anti-inflammatory activity secondary to Ca2+-dependent PLA2 inhibition.
