Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis.
10.4196/kjpp.2010.14.4.205
- Author:
Changyong CHOE
1
;
Jeong Won PARK
;
Eun Suk KIM
;
Sung Gyu LEE
;
Sun Young PARK
;
Jeong Soon LEE
;
Myung Je CHO
;
Kee Ryeon KANG
;
Jaehee HAN
;
Dawon KANG
Author Information
1. Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751, Korea. dawon@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Actin;
Desmin;
Endometritis;
Proteomics
- MeSH:
Actins;
Collectins;
Desmin;
Endometritis;
Endometrium;
Female;
Heat-Shock Proteins;
Hemoglobins;
Interleukin-2;
Potassium Channels;
Proteins;
Proteomics;
Receptors, LH;
RNA, Messenger;
Transferrin
- From:The Korean Journal of Physiology and Pharmacology
2010;14(4):205-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, alpha-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin beta subunit, and potassium channel tetramerisation domain-containing 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, alpha-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and alpha-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and alpha-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.
