Toll-like Receptor3-mediated Induction of Chemokines in Salivary Epithelial Cells.
10.4196/kjpp.2010.14.4.235
- Author:
Jingchao LI
1
;
Mi Young JEONG
;
Ji Hyun BAE
;
Yong Hwan SHIN
;
Meihong JIN
;
Sung Min HANG
;
Jeong Chai LEE
;
Sung Joong LEE
;
Kyungpyo PARK
Author Information
1. Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute, Seoul 110-749, Korea. kppark@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Toll-like receptors;
HSG cells;
Chemokine;
Poly(I:C);
IP-10
- MeSH:
Chemokines;
Chemotaxis;
Culture Media;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
Gene Expression;
Humans;
Lymphocytes;
Real-Time Polymerase Chain Reaction;
T-Lymphocytes;
Toll-Like Receptors
- From:The Korean Journal of Physiology and Pharmacology
2010;14(4):235-240
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(I:C)) significantly increased interferon-gamma-inducible protein 10 (IP-10), interferoninducible T-cell alpha chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.
