Effect of Extracts from Safflower Seeds on Osteoblastic Differentiation and Intracellular Free Calcium Concentration in MC3T3-E1 Cells.
- Author:
Hye Ock JANG
1
;
Hyun Sup EOM
;
Sung Bae ROH
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Korea. iyun@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Safflower seed extracts;
Intracellular free calcium concentration;
MC3T3-E1 cells;
Osteoblastic differentiation;
Alkaline phosphatase activity
- MeSH:
Calcium Channels;
Calcium*;
Carthamus tinctorius*;
Deficiency Diseases;
Osteoblasts*;
Osteoporosis
- From:The Korean Journal of Physiology and Pharmacology
2005;9(1):55-62
- CountryRepublic of Korea
- Language:English
-
Abstract:
Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, 3microliter of 0.1% dried crude extract or 2microliter of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the [Ca2+]i of the cultured osteoblast cells: 3microliter of 0.1% dried crude extract and 2microliter of 0.1% dried aqueous fraction significantly increased the [Ca2+]i of the cultured osteoblast cells (8x104) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the [Ca2+]i of the cultured osteoblast cells, and 300microM Cd2+, specific calcium channel blocker, completely blocked the increase. Therefore, the increased [Ca2+]i of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.
