Protective mechanism of nitric oxide and mucus against ischemia/reperfusion-induced gastric mucosal injury.
- Author:
Hyeyoung KIM
1
;
Kwangsoo NAM
;
Kyung Hwan KIM
Author Information
1. Department of Pharmacology and Institute of Gastroenterology, Yonsei University College of Medicine, Seoul 120 752, South Korea.
- Publication Type:Original Article
- Keywords:
Nitric oxide;
Mucus;
Gastric mucosa;
Ischemia/reperfusion
- MeSH:
Animals;
Arginine;
Celiac Artery;
Constriction;
Gastric Mucosa;
Glutathione;
Glutathione Peroxidase;
Humans;
Mesenteric Artery, Superior;
Microscopy, Electron, Scanning;
Mucus*;
NG-Nitroarginine Methyl Ester;
Nitric Oxide Synthase;
Nitric Oxide*;
Nitroprusside;
Rats;
Reperfusion;
Tissue Donors
- From:The Korean Journal of Physiology and Pharmacology
1998;2(4):511-519
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the role of nitric oxide on the oxidative damage in gastric mucosa of rats which received ischemia/reperfusion and its relation to mucus. Nitric oxide synthesis modulators such as L-arginine and NG-nitro-L-arginine methyl ester, and sodium nitroprusside, a nitric oxide donor, were injected intraperitoneally to the rats 30 min prior to ischemia/reperfusion which was induced by clamping the celiac artery and the superior mesenteric artery for 30 min and reperfusion for 1 h. Lipid peroxide production, the contents of glutathione and mucus, and glutathione peroxidase activities of gastric mucosa were determined. Histological observation of gastric mucosa was performed by using hematoxylin-eosin staining and scanning electron microscopy. The result showed that ischemia/reperfusion increased lipid peroxide production and decreased the contents of glutathione and mucus as well as glutathione peroxidase activities of gastric mucosa. Ischemia/reperfusion induced gastric erosion and gross epithelial disruption of gastric mucosa. Pretreatment of L-arginine, a substrate for nitric oxide synthase, and sodium nitroprusside prevented ischemia/reperfusion-induced alterations of gastric mucosa. However, NG-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor, deteriorated oxidative damage induced by ischemia/reperfusion. In conclusion, nitric oxide has an antioxidant defensive role on gastric mucosa by maintaining mucus, glutathione, and glutathione peroxidase of gastric mucosa.
