Role of phospholipase A2 in oxidant-induced alteration in phosphate transport in primary cultured rabbit renal proximal tubule cells.
- Author:
Kwon Moo PARK
1
;
Sun Hee KO
;
Jae Suk WOO
;
Jin Sup JUNG
;
Sang Ho LEE
;
Yong Keun KIM
Author Information
1. Department of Physiology, College of Medicine, Pusan National University, Pusan 602-739, Korea.
- Publication Type:Original Article
- Keywords:
Phosphate uptake;
Oxidant;
PLA2;
Proximal tubular cells
- MeSH:
Arachidonic Acid;
Cell Survival;
Glucose;
Lipid Peroxidation;
Phospholipases A2*;
Phospholipases*;
Quinacrine;
Staurosporine;
tert-Butylhydroperoxide
- From:The Korean Journal of Physiology and Pharmacology
1998;2(5):601-609
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was undertaken to examine the role of phospholipase A2 (PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose-dependent fashion. A PLA2 inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na+-K+-ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA2 activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.
