Selective cytotoxicity of a novel platinum(II) coordination complex on human bladder cancer cell lines and normal kidney cells.
- Author:
Jee Chang JUNG
1
;
Joo Ho CHUNG
;
Sung Goo CHANG
;
Young Soo RHO
Author Information
1. Department of Pharmacology, School of Medicine, Kyung Hee University, Hoeki-dong, Dongdaemoon-ku, Seoul, South Korea.
- Publication Type:Original Article
- MeSH:
Cell Line*;
Cisplatin;
Glucose;
Humans*;
Kidney*;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:The Korean Journal of Physiology and Pharmacology
2000;4(2):159-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
We have synthesized a novel platinum(II) coordination complex containing cis-1,2-diaminocyclohexane (DACH) as a carrier ligand and 1,2-dichloroethane (DCE) as a leaving group. In addition, nitrate was added to improve the water-solubility. A new series of (Pt(cis-DACH)(DCE)) cntdot 2NO3(PC) was evaluated for its cytotoxic activity on T-24 and J-82 human bladder carcinoma cells and normal primary cultured kidney cells. PC has demonstrated high levels of cytotoxicity against T-24 and J-82 cells. The cytotoxicity of PC against rabbit proximal renal tubular cells, human renal cortical cells and human renal cortical tissues, determined using the MTT assaying technique, the (3H)-thymidine uptake and glucose consumption tests, was found to be quite less than those of cisplatin. Based on these results, this novel platinum(II) coordination complex appears to be better for improving antitumor activities with low nephrotoxicity and is a valuable lead in the development of new clinically available anticancer chemotherapeutic agents.
