Decreasing Effect of Lidocaine.HCl on the Thickness of the Neuronal and Model Membrane.
10.4196/kjpp.2013.17.4.253
- Author:
Sung Min PARK
1
;
Jong Sun PARK
;
Jae Han KIM
;
Jin Hyun BAEK
;
Tae Gyun YOON
;
Do Keun LEE
;
Won Hyang RYU
;
In Kyo CHUNG
;
Uy Dong SOHN
;
Hye Ock JANG
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, School of Dentistry and Research Institute for Oral Biotechnology, Yangsan Campus of Pusan National University, Yangsan 626-870, Korea. jho9612@pusan.ac.kr, iyun@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Fluorescence quenching technique;
Lidocaine.HCl;
Membrane thickness;
Model membranes;
Neuronal membranes
- MeSH:
Anilino Naphthalenesulfonates;
Cell Membrane;
Cerebral Cortex;
Energy Transfer;
Lipid Bilayers;
Liposomes;
Membranes;
Neurons;
Phospholipids;
Propane
- From:The Korean Journal of Physiology and Pharmacology
2013;17(4):253-257
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study examined the mechanism of action of a local anesthetic, lidocaine.HCl. Energy transfer between the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, and the hydrophobic fluorescent probe, 1,3-di(1-pyrenyl) propane, was used to determine the effect of lidocaine.HCl on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex, and liposomes of the total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. The thickness (D) of the intact SPMV, SPMVTL and SPMVPL were 1.044+/-0.008, 0.914+/-0.005 and 0.890+/-0.003 (arbitrary units, n=5) at 37degrees C (pH 7.4), respectively. Lidocaine.HCl decreased the thickness of the neuronal and model membrane lipid bilayers in a dose-dependent manner with a significant decrease in the thickness, even at 0.1 mM. The decreasing effect of lidocaine.HCl on the membrane thickness might be responsible for some, but not all of its anesthetic action.
