H2 Receptor-Mediated Relaxation of Circular Smooth Muscle in Human Gastric Corpus: the Role of Nitric Oxide (NO).
10.4196/kjpp.2014.18.5.425
- Author:
Sang Eok LEE
1
;
Dae Hoon KIM
;
Young Chul KIM
;
Joung Ho HAN
;
Woong CHOI
;
Chan Hyung KIM
;
Hye Won JEONG
;
Seon Mee PARK
;
Sei Jin YUN
;
Song Yi CHOI
;
Rohyun SUNG
;
Young Ho KIM
;
Ra Young YOO
;
Park Hee SUN
;
Heon KIM
;
Young Jin SONG
;
Wen Xie XU
;
Hyo Yung YUN
;
Sang Jin LEE
Author Information
1. Department of Surgery, Konyang University Hospital, Daejeon 302-718, Korea.
- Publication Type:Original Article
- Keywords:
Circular smooth muscle;
Corpus;
H2 receptors;
Histamine;
Human stomach;
Nitric Oxide (NO);
Relaxation
- MeSH:
Apamin;
Glyburide;
Guanylate Cyclase;
Histamine;
Humans;
Muscle, Smooth*;
Nerve Block;
NG-Nitroarginine Methyl Ester;
Nitric Oxide*;
Ranitidine;
Receptors, Histamine H2;
Relaxation*;
Tetraethylammonium
- From:The Korean Journal of Physiology and Pharmacology
2014;18(5):425-430
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was designed to examine the effects of histamine on gastric motility and its specific receptor in the circular smooth muscle of the human gastric corpus. Histamine mainly produced tonic relaxation in a concentration-dependent and reversible manner, although histamine enhanced contractility in a minor portion of tissues tested. Histamine-induced tonic relaxation was nerve-insensitive because pretreatment with nerve blockers cocktail (NBC) did not inhibit relaxation. Additionally, K+ channel blockers, such as tetraethylammonium (TEA), apamin (APA), and glibenclamide (Glib), had no effect. However, N(G)-nitro-L-arginine methyl ester (L-NAME) and 1H-(1,2,4)oxadiazolo (4,3-A) quinoxalin-1-one (ODQ), an inhibitor of soluble guanylate cyclase (sGC), did inhibit histamine-induced tonic relaxation. In particular, histamine-induced tonic relaxation was converted to tonic contraction by pretreatment with L-NAME. Ranitidine, the H2 receptor blocker, inhibited histamine-induced tonic relaxation. These findings suggest that histamine produced relaxation in circular smooth muscle of human gastric smooth muscle through H2 receptor and NO/sGC pathways.
