Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice.
10.4196/kjpp.2014.18.2.183
- Author:
Gui Jie LI
1
;
Peng SUN
;
Rui WANG
;
Ya Lin ZHOU
;
Yu QIAN
;
Xin ZHAO
Author Information
1. Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. foods@live.cn
- Publication Type:Original Article
- Keywords:
Cytokine;
Gastric ulcer;
ICR mice;
Larimichthys crocea swim bladder;
Polysaccharide
- MeSH:
Animals;
Blotting, Western;
Inflammation;
Mice;
Mice, Inbred ICR*;
NF-kappa B;
Ranitidine;
Reserpine*;
Stomach Ulcer*;
Ulcer;
Urinary Bladder*
- From:The Korean Journal of Physiology and Pharmacology
2014;18(2):183-190
- CountryRepublic of Korea
- Language:English
-
Abstract:
This project's aim was to determine the reserpine-induced gastric ulcer preventive effect of polysaccharide of Larimichthys crocea swim bladder (PLCSB) in ICR mice. The anti-gastric ulcer effects of polysaccharide of Larimichthys crocea swim bladder was evaluated in mice model using morphological test, serum levels assay, cytokine levels assay, tissue contents analysis, reverse transcription-polymerase chain reaction (RT-PCR) analysis and western bolt assay. High concentration (50 mg/kg dose) of PLCSB reduced IFN-gamma as compared to low concentration (25 mg/kg dose) and control mice. SS and VIP serum levels of PLCSB treated mice were higher than those of control mice, and MOT and SP serum levels were lower than control mice. Gastric ulcer inhibitory index of PLCSB treatment groups mice were much lower than control mice, and the high concentration treated mice were similar to the ranitidine treated mice. The SOD and GSH-Px activities of PLCSB treated mice were higher than control mice, close to normal mice and ranitidine treated mice. PLCSB treated mice also showed the similar contents of NO and MDA to normal group. By RT-PCR and western blot assay, PLCSB significantly induced inflammation in tissues of mice by downregulating NF-kappaB, iNOS, and COX-2, and upregulating IkappaB-alpha . These results suggest that PLCSB showed a good gastric ulcer preventive effect as the gastric ulcer drug of ranitidine. Polysaccharide of Larimichthys crocea swim bladder may be used as a drug material from marine products.
