Effects of Local Anesthetics on Rotational Mobility of n- (9-Anthroyloxy) stearic Acid in Neuronal Membranes.
- Author:
Hye Ock JANG
1
;
Chang LEE
;
Min Gak CHOI
;
Sang Hun SHIN
;
In Kyo CHUNG
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Korea. iyun@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Fluorescent probe technique;
Rotational mobility;
Hydrocarbon interior;
Local anesthetics;
Neuronal membranes;
AS probes
- MeSH:
Anesthetics, Local*;
Anisotropy;
Bupivacaine;
Carbon;
Cell Membrane;
Fluorescence Polarization;
Lidocaine;
Membrane Fluidity;
Membranes*;
Neurons*;
Prilocaine;
Procaine;
Tetracaine
- From:The Korean Journal of Physiology and Pharmacology
2003;7(3):119-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
To elucidate the molecular mechanism of pharmacological action of local anesthetics, we studied membrane actions of tetracaine, bupivacaine, lidocaine, prilocaine and procaine. Fluorescence polarization of n- (9-anthroyloxy) stearic acid (n-AS) was used to examine the effects of these local anesthetics on differential rotational mobility of different positions of the number of synaptosomal plasma membrane vesicle (SPMV) phospholipid carbon atoms. The four membrane components differed with respect to 3, 6, 9 and 16- (9-anthroyloxy) stearic acid (3-AS, 6-AS, 9-AS and 16-AP) probes, indicating that differences in the membrane fluidity might be present. Degrees of the rotational mobility of 3-AS, 6-AS, 9-AS and 16-AP were different depending on depth of hydrocarbon interior. In a dose-dependent manner, tetracaine, bupivacaine, lidocaine, prilocaine and procaine decreased anisotropy of 3-AS, 6-AS, 9-AS and 16-AP in the hydrocarbon interior of the SPMV. These results indicate that local anesthetics have significant disordering effects on hydrocarbon interior of the SPMV, thus affecting the transport of Na+ and K+ in nerve membranes and leading to anesthetic action.
