Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder.
10.4196/kjpp.2013.17.6.531
- Author:
Sun Ouck KIM
1
;
Han Seong JEONG
;
Sujeong JANG
;
Mei Jin WU
;
Jong Kyu PARK
;
Han Yi JIAO
;
Jae Yeoul JUN
;
Jong Seong PARK
Author Information
1. Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea. parkjs@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Interstitial cells of Cajal;
Spontaneous electrical activities;
Urinary bladder
- MeSH:
Action Potentials;
Adenosine Triphosphate;
Animals;
Atropine;
Carbachol;
Interstitial Cells of Cajal*;
Membranes;
Mice*;
Muscle, Smooth;
Neurotransmitter Agents;
Phenotype;
Urinary Bladder*
- From:The Korean Journal of Physiology and Pharmacology
2013;17(6):531-536
- CountryRepublic of Korea
- Language:English
-
Abstract:
Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca2+ ([Ca2+]i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca2+]i oscillations and basal Ca2+ levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.
