Molecular Characterization of Nippostrongylus brasiliensis (Nematoda: Heligmosomatidae) from Mus musculus in India.
10.3347/kjp.2016.54.6.743
- Author:
Anshu CHAUDHARY
1
;
Urvashi GOSWAMI
;
Hridaya Shanker SINGH
Author Information
1. Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), 250004, India. goswamiurvashi12@gmail.com
- Publication Type:Original Article
- Keywords:
Nippostrongylus brasiliensis;
Mus musculus;
nematode;
Meerut;
Uttar Pradesh;
mitochondrial cox1;
protein
- MeSH:
Ancylostomatoidea;
Animals;
Biochemistry;
Databases, Nucleic Acid;
Electron Transport Complex IV;
India*;
Mice*;
Microscopy, Electron, Scanning;
Nippostrongylus*;
Polymerase Chain Reaction;
Rodentia;
Sequence Analysis
- From:The Korean Journal of Parasitology
2016;54(6):743-750
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mus musculus (Rodentia: Muridae) has generally been infected with a rodent hookworm Nippostrongylus brasiliensis. In this report, we present morphological and molecular identification of N. brasiliensis by light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the protein sequences encoded by cox1 gene, respectively. Despite the use of N. brasiliensis in many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis of cox1 gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species is N. brasiliensis. This study represents the first record of molecular identification of N. brasiliensis from India and the protein structure to better understand the comparative phylogenetic characteristics.
