α-Isocubebene modulates vascular tone by inhibiting myosin light chain phosphorylation in murine thoracic aorta.
10.4196/kjpp.2018.22.4.437
- Author:
Byeong Hyeok YE
1
;
Eun Jung KIM
;
Seung Eun BAEK
;
Young Whan CHOI
;
So Youn PARK
;
Chi Dae KIM
Author Information
1. Department of Pharmacology, School of Medicine, Pusan National University, Yangsan 50612, Korea. chidkim@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
α-Iso-cubebene;
Myosin light chain;
Vascular tone;
Vascular smooth muscle cells
- MeSH:
Animals;
Aorta, Thoracic*;
Calcium;
Cytosol;
Endothelium;
Hypertension;
Lignin;
Mice;
Muscle, Smooth, Vascular;
Myosin Light Chains*;
Myosins*;
Norepinephrine;
Phenylephrine;
Phosphorylation*;
Plants, Medicinal;
Rats;
Relaxation;
Schisandra
- From:The Korean Journal of Physiology and Pharmacology
2018;22(4):437-445
- CountryRepublic of Korea
- Language:English
-
Abstract:
α-Iso-cubebene (ICB) is a dibenzocyclooctadiene lignin contained in Schisandra chinensis (SC), a well-known medicinal herb that ameliorates cardiovascular symptoms, but the mechanism responsible for this activity has not been determined. To determine the role played by ICB on the regulation of vascular tone, we investigated the inhibitory effects of ICB on vascular contractile responses by adrenergic α-receptor agonists. In addition, we investigated the role on myosin light chain (MLC) phosphorylation and cytosolic calcium concentration in vascular smooth muscle cells (VSMC). In aortic rings isolated from C57BL/6J mice, ICB significantly attenuated the contraction induced by phenylephrine (PE) and norepinephrine (NE), whereas ICB had no effects on KCl (60 mM)-induced contraction. In vasculatures precontracted with PE, ICB caused marked relaxation of aortic rings with or without endothelium, suggesting a direct effect on VSMC. In cultured rat VSMC, PE or NE increased MLC phosphorylation and increased cytosolic calcium levels. Both of these effects were significantly suppressed by ICB. In conclusion, our results showed that ICB regulated vascular tone by inhibiting MLC phosphorylation and calcium flux into VSMC, and suggest that ICB has anti-hypertensive properties and therapeutic potential for cardiovascular disorders related to vascular hypertension.
