DHA and EPA Down-regulate COX-2 Expression through Suppression of NF-kappa B Activity in LPS-treated Human Umbilical Vein Endothelial Cells.
10.4196/kjpp.2009.13.4.301
- Author:
Soon Ae LEE
1
;
Hye Jung KIM
;
Ki Churl CHANG
;
Jong Chul BAEK
;
Ji Kwon PARK
;
Jeong Kyu SHIN
;
Won Jun CHOI
;
Jong Hak LEE
;
Won Young PAIK
Author Information
1. Department of Obstetrics & Gynecology, School of Medicine, Gyeongsang National University, Jinju 660-702, Korea. wypaik@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
DHA;
EPA;
Cyclooxygenase-2;
Nuclear factor-kappa B;
Endothelium
- MeSH:
Blotting, Western;
Chromans;
Cyclooxygenase 2;
Dinoprostone;
Eicosapentaenoic Acid;
Endothelial Cells;
Endothelium;
Enzyme-Linked Immunosorbent Assay;
Human Umbilical Vein Endothelial Cells;
Humans;
Interleukin-6;
Luciferases;
NF-kappa B;
Thiazolidinediones
- From:The Korean Journal of Physiology and Pharmacology
2009;13(4):301-307
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioprotective potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin E2 (PGE2) and IL-6 production, and NF-kappaB activity were measured by Western blot, ELISA, and luciferase activity, respectively. Results showed that EPA, DHA, or troglitazone significantly reduced COX-2 expression, NF-kappaB luciferase activity, and PGE2 and IL-6 production in a dose-dependent fashion. Interestingly, low doses (10 micrometer) of DHA and EPA, but not troglitozone, significantly increased the activity of NF-kappaB in resting HUVECs. Our study suggests that while DHA, EPA, and troglitazone may be protective on HUVECs under inflammatory conditions in a dose-dependent manner. However there may be some negative effects when the concentrations are abnormally low, even in normal endothelium.
