Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase.
10.4196/kjpp.2009.13.6.431
- Author:
Kwon Ho LEE
1
;
Sang Ki LEE
;
Hyo Shin KIM
;
Eun Jung CHO
;
Hee Kyoung JOO
;
Eun Ji LEE
;
Ji Young LEE
;
Myoung Soo PARK
;
Seok Jong CHANG
;
Chung Hyun CHO
;
Jin Bong PARK
;
Byeong Hwa JEON
Author Information
1. Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Korea. bhjeon@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1);
Lead;
Cell death;
Endothelial cells;
Catalase
- MeSH:
Catalase;
Cell Death;
Down-Regulation;
Endothelial Cells;
Hydrogen Peroxide;
Superoxides;
Up-Regulation
- From:The Korean Journal of Physiology and Pharmacology
2009;13(6):431-436
- CountryRepublic of Korea
- Language:English
-
Abstract:
The role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the lead (Pb)-induced cellular response was investigated in the cultured endothelial cells. Pb caused progressive cellular death in endothelial cells, which occurred in a concentration- and time-dependent manner. However, Ref-1 overexpression with AdRef-1 significantly inhibited Pb-induced cell death in the endothelial cells. Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Pb exposure induced the downregulation of catalase, it was inhibited by the Ref-1 overexpression in the endothelial cells. Taken together, our data suggests that the overexpression of Ref-1 inhibited Pb-induced cell death via the upregulation of catalase in the cultured endothelial cells.
