Type II and III Taste Bud Cells Preferentially Expressed Kainate Glutamate Receptors in Rats.
10.4196/kjpp.2009.13.6.455
- Author:
Sang Bok LEE
1
;
Cil Han LEE
;
Se Nyun KIM
;
Ki Myung CHUNG
;
Young Kyung CHO
;
Kyung Nyun KIM
Author Information
1. Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, Korea. knkim@gwnu.ac.kr
- Publication Type:Original Article
- Keywords:
Taste bud;
Kainate receptor;
RT-PCR;
Single isolated cell
- MeSH:
Animals;
Cobalt;
Epithelium;
Glutamic Acid;
Kainic Acid;
Rats;
Rats, Sprague-Dawley;
Receptors, Glutamate;
Receptors, Kainic Acid;
RNA, Messenger;
Taste Buds;
Tongue
- From:The Korean Journal of Physiology and Pharmacology
2009;13(6):455-460
- CountryRepublic of Korea
- Language:English
-
Abstract:
Glutamate-induced cobalt uptake reveals that non-NMDA glutamate receptors (GluRs) are present in rat taste bud cells. Previous studies involving glutamate induced cobalt staining suggest this uptake mainly occurs via kainate type GluRs. It is not known which of the 4 types of taste bud cells express subunits of kainate GluR. Circumvallate and foliate papillae of Sprague-Dawley rats (45~60 days old) were used to search for the mRNAs of subunits of non-NMDA GluRs using RT-PCR with specific primers for GluR1-7, KA1 and KA2. We also performed RT-PCR for GluR5, KA1, PLCbeta2, and NCAM/SNAP 25 in isolated single cells from taste buds. Taste epithelium, including circumvallate or foliate papilla, express mRNAs of GluR5 and KA1. However, non-taste tongue epithelium expresses no subunits of non-NMDA GluRs. Isolated single cell RT-PCR reveals that the mRNAs of GluR5 and KA1 are preferentially expressed in Type II and Type III cells over Type I cells.
