Upregulation of Renin-angiotensin, Endothelin and C-type Natriuretic Peptide in Rat Glomerulus with Bilateral Ureteral Obstruction.
- Author:
Eun Hui BAE
1
;
Soo Wan KIM
Author Information
1. Department of Internal Medicine Chonnam National University Medical School, Gwangju, Korea. skimw@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Bilateral ureteral obstruction;
Renin-angiotensin system;
Aldosterone synthase;
C-type natriuretic peptide;
Endothelin;
Glomerulus
- MeSH:
Aldosterone Synthase;
Animals;
Endothelin-1;
Endothelins*;
Humans;
Ligation;
Male;
Natriuretic Peptide, C-Type*;
Peptidyl-Dipeptidase A;
Rats*;
Rats, Sprague-Dawley;
Real-Time Polymerase Chain Reaction;
Receptors, Angiotensin;
Renin;
Renin-Angiotensin System;
RNA, Messenger;
Up-Regulation*;
Ureter*;
Ureteral Obstruction*
- From:The Korean Journal of Physiology and Pharmacology
2006;10(6):343-347
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was designed to investigate the effects renin-angiotensin-aldosterone system (RAAS), endothelin (ET) and local natriuretic peptide (NP) system for glomerulopathy induced in the experimental bilateral ureteral obstructive rats. Sprague-Dawley male rats (200~220 g body weight) were bilaterally obstructed by ligation of the proximal ureters for 24 hours. Control rats were treated in the same ways, except that no ligature was made. The glomeruli were isolated from cortex by graded sieve methods, and the mRNA expressions of local renin-angiotensin system (RAS), aldosterone synthase (CYP11B2), endothelin-1 (ET-1) and NP system were determined by real-time polymerase chain reaction. Following the bilateral ureteral obstruction, the mRNA expressions of renin, angiotensin converting enzyme 1 as well as ET-1 were increased, while that of angiotensin converting enzyme 2 was not changed. The expressions of CYP11B2 and angiotensin II receptors were not changed. C-type natriuretic peptide (CNP) expression was increased, while its receptors (natriuretic peptide receptor-B) were not changed. We suggest that the upregulation of local RAS and ET play a role in the progressive glomerular injury, and that the enhanced CNP activity also plays a compensatory role in obstructive uropathy in the glomerulus.
