Coordinate expression of renin and cyclooxygenase-2 in rats with two-kidney, one clip and deoxycorticosterone acetate-salt hypertension.
- Author:
Jong Un LEE
1
;
Yoon Wha OH
;
Sunmi KIM
Author Information
1. Department of Physiology, Chonnam National University Medical School,South Korea. julee@chonnam.ac.kr
- Publication Type:Original Article
- MeSH:
Animals;
Blood Pressure;
Cyclooxygenase 2*;
Desoxycorticosterone*;
Hand;
Humans;
Hypertension*;
Kidney;
Male;
Rats*;
Rats, Sprague-Dawley;
Renin*;
Renin-Angiotensin System;
RNA, Messenger
- From:The Korean Journal of Physiology and Pharmacology
2001;5(3):253-258
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was aimed to examine whether the expression of renin is associated with that of cyclooxygenase-2 (COX-2) in the kidney. Male Sprague-Dawley rats were made two-kidney, one clip (2K1C) or deoxycorticosterone acetate (DOCA)-salt hypertensive, to stimulate or to inhibit the endogenous renin-angiotensin system, respectively. The expression of renin and COX-2 mRNA was determined in the cortex of the kidney by reverse transcription-polymerase chain reaction. 2K1C hypertensive rats showed an increased expression of renin as well as of COX-2 in the clipped kidney. The expression of renin was decreased in parallel with that of COX-2 in the contralateral non-clipped kidney. Removal of the renal arterial clip reversed the expression of both genes, along with the blood pressure, to the control level. On the other hand, DOCA-salt hypertension was associated with parallel decreases of renin and COX-2 expression. These results indicate that renin and COX-2 genes are coordinately expressed in the kidney.
