Caffeic Acid Phenethyl Ester Inhibits the PKC-Induced IL-6 Gene Expression in the Synoviocytes of Rheumatoid Arthritis Patients.
- Author:
Gang Min HUR
1
;
Yin Bang HWANG
;
Jae Heun LEE
;
So Hyun BAE
;
Ji Sun PARK
;
Choong Jae LEE
;
Jeong Ho SEOK
Author Information
1. Department of Pharmacology, College of Medicine, Chungnam National University, Daejon 301-747, South Korea. jhseok@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
IL-6 gene expression;
Phorbol ester;
Caffeic acid phenethyl ester;
Synoviocytes;
Rheumatoid arthritis
- MeSH:
Arthritis, Rheumatoid*;
Cytokines;
Gene Expression*;
Humans;
Interleukin-6*;
RNA;
RNA, Messenger;
Transcription Factors
- From:The Korean Journal of Physiology and Pharmacology
2003;7(6):363-368
- CountryRepublic of Korea
- Language:English
-
Abstract:
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA) -induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, 100 microM) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors (NF-kappaB, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
