Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells.
10.4196/kjpp.2010.14.6.427
- Author:
Ho Sun SONG
1
;
Hee Rae KIM
;
Myoung Soo KO
;
Jae Min JEONG
;
Yong Ho KIM
;
Myung Cheul KIM
;
Yeon Hee HWANG
;
Uy Dong SOHN
;
Yoon Myoung GIMM
;
Sung Ho MYUNG
;
Sang Soo SIM
Author Information
1. College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. simss@cau.ac.kr
- Publication Type:Original Article
- Keywords:
EMF;
Phospholipase A2;
Arachidonic acid;
Phospholipase C;
Phospholipase D
- MeSH:
Arachidonic Acid;
Cell Line;
Cell Membrane;
Cells, Cultured;
Electromagnetic Fields;
Magnets;
Occupational Exposure;
Phospholipase D;
Phospholipases;
Phospholipases A2;
Pyridoxal;
Signal Transduction;
Type C Phospholipases
- From:The Korean Journal of Physiology and Pharmacology
2010;14(6):427-433
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 microM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA2 assay, we failed to observe the change of cPLA2 and sPLA2 activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
