Characteristics of K+ Outward Currents in the Cochlear Outer Hair Cells of Circling Mice within the First Postnatal Week.
10.4196/kjpp.2015.19.4.383
- Author:
Ji Woong AHN
1
;
Shin Wook KANG
;
Seung Cheol AHN
Author Information
1. Department of Physiology, College of Medicine, Dankook University, Cheonan 330-714, Korea. ansil67@hanmail.net
- Publication Type:Original Article
- Keywords:
Circling mice;
Deafness;
DFNB6;
K+ current;
Outer hair cell
- MeSH:
Animals;
Deafness;
Genotype;
Hair*;
Humans;
Mice*;
Models, Animal;
Potassium Channels, Calcium-Activated;
Tea
- From:The Korean Journal of Physiology and Pharmacology
2015;19(4):383-388
- CountryRepublic of Korea
- Language:English
-
Abstract:
K+ outward currents in the outer hair cells (OHCs) of circling mice (homozygous (cir/cir) mice), an animal model for human deafness (DFNB6 type), were investigated using a whole cell patch clamp technique. Littermate heterozygous (+/cir) mice of the same age (postnatal day (P) 0 -P6) were used as controls. Similar slow rising K+ currents were observed in both genotypes, but their biophysical and pharmacological properties were quite different. The values of V(half) for activation were significantly different in the heterozygous (+/cir) and homozygous (cir/cir) mice (-8.1+/-2.2 mV, heterozygous (+/cir) mice (n=7) and -17.2+/-4.2 mV, homozygous (cir/cir) mice (n=5)). The inactivation curve was expressed by a single first order Boltzmann equation in the homozygous (cir/cir) mice, while it was expressed by a sum of two first order Boltzmann equations in the heterozygous (+/cir) mice. The K+ current of homozygous (cir/cir) mice was more sensitive to TEA in the 1 to 10 mM range, while the 4-AP sensitivities were not different between the two genotypes. Removal of external Ca2+ did not affect the K+ currents in either genotype, indicating that the higher sensitivity of K+ current to TEA in the homozygous (cir/cir) mice was not due to an early expression of Ca2+ activated K+ channels. Our results suggest that the K+ outward current of developing homozygous (cir/cir) mice OHCs is different in both biophysical and pharmacological aspects than that of heterozygous (+/cir) mice.
