Calcium Sensitization Induced by Sodium Fluoride in Permeabilized Rat Mesenteric Arteries.
10.4196/kjpp.2010.14.1.51
- Author:
Enyue YANG
1
;
Joon Yong CHO
;
Uy Dong SOHN
;
In Kyeom KIM
Author Information
1. Department of Pharmacology, Kyungpook National University School of Medicine, Daegu 700-422, Korea. inkim@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Calcium sensitization;
Permeabilization;
Mesenteric artery;
Sodium fluoride;
Rho kinase
- MeSH:
Amides;
Animals;
Azepines;
Calcium;
Contracts;
Escin;
Indoles;
Mesenteric Arteries;
Naphthalenes;
Passive Cutaneous Anaphylaxis;
Pyridines;
Rats;
rho-Associated Kinases;
Sodium;
Sodium Fluoride
- From:The Korean Journal of Physiology and Pharmacology
2010;14(1):51-57
- CountryRepublic of Korea
- Language:English
-
Abstract:
It was hypothesized that NaF induces calcium sensitization in Ca2+-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with beta-escin and subjected to tension measurement. NaF potentiated the concentration-response curves to Ca2+ (decreased EC50 and increased E(max)). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascular tension in Ca2+-controlled solution at pCa 7.0 or pCa 6.5, but not at pCa 8.0. NaF-induced vasocontraction and GTPgammaS-induced vasocontraction were not additive. NaF-induced vasocontraction at pCa 7.0 was inhibited by pretreatment with Rho kinase inhibitors H1152 or Y27632 but not with a MLCK inhibitor ML-7 or a PKC inhibitor Ro31-8220. NaF induces calcium sensitization in a Ca2+-dependent manner in beta-escin-permeabilized rat mesenteric arteries. These results suggest that NaF is an activator of the Rho kinase signaling pathway during vascular contraction.
