Activation of Transcription Factor NF-kappaB and c-Jun/AP-1 by Various Compositions of Particles in Osteoclast Precursor Cells.
10.5371/jkhs.2008.20.3.209
- Author:
Yong Sik KIM
1
;
Soon Yong KWON
;
Suk Ku HAN
;
Jae Young KWON
;
Nam Yong CHOI
Author Information
1. Department of Orthopaedic surgery, Kangnam St. Mary's Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Transcription factor;
NF-kappaB;
c-Jun/AP-1;
Particle;
Osteoclast precursor cell;
Electrophoretic mobility shift assay
- MeSH:
Animals;
Arthroplasty, Replacement, Hip;
DNA;
Electrophoretic Mobility Shift Assay;
Mice;
Mice, Inbred C57BL;
NF-kappa B;
Osteoclasts;
Osteolysis;
Polymethyl Methacrylate;
Polystyrenes;
Titanium;
Transcription Factors
- From:Journal of the Korean Hip Society
2008;20(3):209-214
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was performed in order to investigate the effects of various particle preparations on NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells. MATERIALS AND METHODS: Osteoclast precursor cells isolated from C57BL mice were treated with PMMA (polymethylmethacrylate) spheres, polystyrene, titanium particles, and retrieved metal particles from failed cementless total hip replacements. NF-kappaB and c-Jun/AP-1 DNA binding activities were analyzed using electrophoretic mobility shift assays (EMSA). RESULTS: Commercially available PMMA and polystyrene spheres routinely showed negativity on endotoxin assays, but titanium particles and retrieved metal particles consistently showed positivity. PMMA spheres, with a maximal response noted at 30 minutes with an optimal concentration of 0.6 mg/ml, were potent stimulator of NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells. Other particles (polystyrene, titanium, metal retrievals) also activated transcription factor NF-kappaB and c-Jun/AP-1 compared to controls. Endotoxin removal from retrieved metal particles diminished the biologic effect by approximately 40%. CONCLUSION: Particles of various compositions and sizes (PMMA, polystyrene, titanium, and retrieved metal particles) activated the NF-kappaB and c-Jun/AP-1 signaling pathways. This suggests that NF-kappaB and c-Jun/AP-1 may have important roles in the pathogenesis of periprosthetic osteolysis.
