Potential Role of Homer-2a on Cutaneous Vascular Anomaly.
10.3346/jkms.2002.17.5.636
- Author:
Jeong Tae KIM
1
;
Si Hyun PARK
;
Soek Kwun KIM
;
Eun Young KWON
;
Mi Hyang DO
;
Tae Ho HWANG
Author Information
1. Department of Plastic and Reconstructive Surgery, Dong-A University, Busan, Korea. thhwang@mail.donga.ac.kr
- Publication Type:Original Article
- Keywords:
Homer-2a;
Vascular Anomaly;
Human Umbilical Vein Endothelial Cells
- MeSH:
Adolescent;
Adult;
Animals;
Apoptosis;
Base Sequence;
Blood Vessels/*abnormalities;
Carrier Proteins/genetics/*physiology;
Cells, Cultured;
Child;
Child, Preschool;
DNA, Complementary/genetics;
Endothelium, Vascular/cytology;
Female;
Hemangioma/blood supply/*genetics;
Humans;
Male;
Mice;
Middle Aged;
Neuropeptides/genetics/*physiology;
Rats;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/blood supply;
Skin Neoplasms/blood supply/*genetics
- From:Journal of Korean Medical Science
2002;17(5):636-640
- CountryRepublic of Korea
- Language:English
-
Abstract:
Homer protein was identified based on its rapid induction in rat hippocampal granule cell neurons following excitatory synaptic activity. Although the presence of the Homer gene in the peripheral tissues has been observed in previous reports, the physiological function of the Homer protein in these tissues has not been noted. In this experiment, a Homer-2a cDNA fragment was successfully amplified by RTPCR in the involuting phase of human hemangioma but not in the human vascular malformation and normal vessel. After isolation of full Homer cDNA in a mouse liver cDNA library, E1-deleted recombinant adenovirus expressing the Homer protein (Adv.CMV.mHomer-2a) was constructed to determine its physiological function in peripheral tissues. Adv.CMV.mHomer2a, but not Adv.CMV.LacZ (recombinant adenovirus expressing beta-galactosidase), strongly inhibited the growth rate of HUVECs (human umbilical vein endothelial cells) probably via inducing apoptosis determined by acridine orange/ethidium bromide (AO/EB) staining methods. This study suggests that the Homer gene is present in human specimens in the involuting phase of hemangioma, and it might be involved in the growth control.
