Role of Protein Kinase C-delta in Atherosclerosis.
10.5758/kjves.2011.27.2.61
- Author:
Sang Jun PARK
1
;
Jong Pil JUNG
;
Hye Jeong KIM
;
Eun A LEE
;
Tae Won KWON
;
Byungsuk KWON
;
Hong Rae CHO
Author Information
1. Department of Surgery, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. hrcho@uuh.ulsan.kr
- Publication Type:Original Article
- Keywords:
Protein kinase C delta;
Atherosclerosis
- MeSH:
Animals;
Aorta;
Apoptosis;
Atherosclerosis;
Cell Differentiation;
Chemokine CCL3;
Inflammation;
Interleukin-2;
Mice;
Protein Kinase C;
Protein Kinase C-delta;
Protein Kinases;
RNA, Messenger;
Transplantation, Homologous;
Transplants
- From:Journal of the Korean Society for Vascular Surgery
2011;27(2):61-65
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Protein kinase C (PKC) has been implicated in a wide variety of cellular processes. Although PKC-delta is implicated in cell growth inhibition, as well as in cell differentiation, apoptosis, and tumor suppression, its role in atherosclerosis remains unclear. This study aimed to identify the mechanism of PKC-delta in the development of atherosclerosis. METHODS: To induce atherosclerosis, we performed allograft transplantations on aortas in mice. At 2, 4, and 6 weeks after transplantation, grafted aortas were obtained to compare the degree of atherosclerosis between wild type and PKC-delta (-/-) aorta. Alloantibody levels in the recipient mice's blood were measured. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to quantitatively measure chemokine and cytokine mRNA expression of the inflammation from the harvested aorta. RESULTS: Atherosclerosis was more severe in the PKC-delta (-/-) aorta than in the wild type aorta. Alloantibody levels were higher in the mice grafted with aorta from the PKC-delta (-/-) mice than in the mice grafted with aorta from the wild type mice. RT-PCR revealed higher expressions of MRP-2, MCP-1, MIP-1alpha, and IL-2 in the mice grafted with aorta from the PKC-delta (-/-) mice than the wild type mice. CONCLUSION: Aorta allograft transplantation is a useful modality for inducing atherosclerosis. PKC-delta may be a negative regulator of atherosclerosis.
