Immunocytochemical Characteristics of the Short-term cultured Mesothelial Cells.
- Author:
Ho Jong JEON
1
;
Mi Ja LEE
;
Mi Sook LEE
;
Yu Kyung JEONG
;
Young Mi LEE
;
Hyung Ho CHOI
Author Information
1. Departments of Pathology, College of Medicine, Chosun University, Korea.
- Publication Type:Original Article
- Keywords:
Resting and reactive mesothelial cells;
Immununocytochemical stain;
Short-term culture
- MeSH:
Actins;
Antibodies;
Cytoskeleton;
Desmin;
Exudates and Transudates;
Hot Temperature;
Humans;
Intermediate Filaments;
Keratins;
Liver Cirrhosis;
Mucin-1;
Muramidase;
Pleural Effusion;
Vimentin
- From:Korean Journal of Cytopathology
1995;6(2):106-115
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Reactive human mesothelial cells were examined by immunocytochemical stain with intermediate filaments(cytokeratin [CK1, CK7, CK8, CK18, CD19/, vimentin, desmin, actin), epithelial membrane antigen, carcinoembryonic antigen(CEA), MHC class II antigen(HLA-DR), LeuM-1(CD15), alpha1-antitrypsin(ACT), alpha1-antichymotrypsin (ACHT), CD68(KP-1) and FcgammaRIII(CD16). The mesothelial cells were isolated from patients with liver cirrhosis and pleural effusion, and short-term cultured in RPMI 1640 media containing 10% heat inactivated fetal calf serum and 1% identical supernatant fluid of the patients' transudates. The results obtained are as follows. 1. The cultured-reactive mesothelial cells were positive for the protein of cytoskeleton such as cytokeratin and vimentin, but negative for desmin and actin. The resting mesothelial cells showed positive reactions for cytokeratin, but negative for vimentin, desmin and actin. 2. The primary antibodies to the cytokeratin were strongly reactive for CK1, CK8 and CK18 but negative for CK7 and CK19 in both reactive and resting mesothelial cells. 3. Resting mesothelial cells showed negative reactions for CEA, but strong positive reactions in cultured-reactive mesothelial cells. 4. The markers for the monocytes\histiocytes (CD11b, CD14, CD16, CD68, lysozyme and alpha1-antitrypsin and alpha1-antichymotrypsin) were nonreactive in resting mesothelial cells, but lysozyme and alpha1-antitrypsin were weakly reactive in reactive and proliferative mesothelial cells. 5. MHC Class II molecule(HLA-DR antigen) was negative in both resting and reactive mesothelial cells. These results suggest that the short-term cultured, reactive mesothelial cells show a newly aberrant expression of the vimentin and carcino-embryonic antigen. The reason of the aberrant expression of the intermediate filament and oncofetal antigen in reactive and proliferative mesothelial cells should be further evaluated.
