Immunocytochemical Detection of Pneumocystis Carinii in Bronchoalveolar Lavage.
- Author:
Kun Young KWON
1
;
Seung Che CHO
;
Sang Pyo KIM
;
Kwan Kyu PARK
;
Eun Sook CHANG
;
Chung Sook KIM
Author Information
1. Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine.
- Publication Type:Original Article
- Keywords:
Pneumocystis carinii;
Gomori methenamine silver;
Diff-Quik;
Immunocy- tochemical staining;
Bronchoalveolar lavage
- MeSH:
Bronchoalveolar Lavage*;
Coloring Agents;
Diagnosis;
Lung;
Methenamine;
Pneumocystis carinii*;
Pneumocystis*;
Pneumonia
- From:Korean Journal of Cytopathology
1997;8(1):27-34
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Pneumocystis carinii is an established cause of pulmonary infections in immuno- compromised hosts. Several cytological stains, such as Papanicolaou, Gomori methenamine silver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochemical stains that recognize P. carinii in bronchoalveolar lavage from experimentally induced P. carinii pneumonia rats(n=15). In addition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected in 9 of 10 cases (90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in all cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool in addition to GMS and Diff-Quik stain to detect P. carinii organisms in bronchoalveolar lavage.
