The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates.
- Author:
Tae Jung KIM
1
;
Jae Il LEE
Author Information
- Publication Type:Original Article
- Keywords: Brachyspira spp.; PCR-RFLP; 23S rRNA; spirochete
- MeSH: Animals; DNA, Bacterial/genetics; Dysentery, Bacillary/diagnosis/microbiology/*veterinary; Korea; Phylogeny; Polymerase Chain Reaction/methods/*veterinary; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 23S/chemistry/*genetics; Spirochaetales/*genetics/*isolation&purification; Spirochaetales Infections/diagnosis/microbiology/*veterinary; Swine; Swine Diseases/diagnosis/*microbiology
- From:Journal of Veterinary Science 2006;7(3):277-280
- CountryRepublic of Korea
- Language:English
- Abstract: Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
