Cell Beath Induced by Ethanol : Prevention of Cell Death by the bcl-2 Proto-Oncogene.
- Author:
Eun Jeong LIM
;
Kyoung Ja HONG
;
Byung Hwan YANG
;
Young Gyu CHAI
- Publication Type:Original Article
- Keywords:
Bcl-2;
Ethanol;
Apoptosis;
ICE;
CPP32;
PARP
- MeSH:
Apoptosis;
Cell Death*;
Cell Line;
DNA Fragmentation;
Ethanol*;
Ice;
Proto-Oncogenes*
- From:Journal of the Korean Society of Biological Psychiatry
1997;4(2):211-218
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The Bcl-2 protein has been shown to block apoptosis induced by a variety of stimuli. We have performed the experiments which cell death can be blocked by the bcl-2 proto-oncogene under moderate(50-100mM) or high ethanol treatment(400-600mM). As a result of morphological changes, and MTT assay, cell death was blocked by Bcl-2 under 100mM ethanol. However, the results of DNA fragmentation and RT-PCR(ICE, and CPP32), immunoblotting(CPP32, and PARP) for SK-pcDNA3 cells(vector only) and SK-Bcl-2 cells(stably expressed bcl-2 gene) were showen to be no significant differences between two cell lines. These result suggested that cell death induced by ethanol was not followed by apoptosis mechanism, and was blocked by the bcl-2 proto-oncogene with moderate ethanol.
