Effect of Antipsychotic Drugs on Dopamine Transporter Function in CV(bDAT) Cells.
- Author:
Young Mi KIM
;
Yong Sung LEE
;
Sang Hun LEE
;
Dong Yul OH
;
Byung Hwan YANG
;
Suck Shin CHO
;
Jung Hyun NAM
- Publication Type:Original Article
- Keywords:
Dopamine transporter;
Dopamine uptake;
Antipsychotic drug
- MeSH:
Antipsychotic Agents*;
Cell Line;
DNA, Complementary;
Dopamine Plasma Membrane Transport Proteins*;
Dopamine*;
Dopaminergic Neurons;
Inhibitory Concentration 50;
Transfection
- From:Journal of the Korean Society of Biological Psychiatry
1997;4(1):48-53
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
CV(bDAT) cell line, expressing dopamine transporter stably, has been established by transfection of CV-1 cells with bovine dopamine transporter cDNA. Using CV(bDAT) cells, the effects of various antipsychotic drugs on dopamine uptake were investigated. All of antipsychotic drugs tested, inhibited the [3>H]dopamine uptake into CV(bDAT) cells with IC50s in the low to mid micromolar, implying that antipsychotic drugs may produce overflow of dopamine in the synaptic cleft of dopaminergic neuron.
