Effect of Antioxidants on Aminoglycoside-induced Cochlear Cell Damage.
- Author:
Sung Il NAM
1
Author Information
1. Department of Otolaryngology, Keimyung University School of Medicine, Daegu, Korea. entnamsi@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Ototoxicity;
Aminoglycoside;
Antioxidant
- MeSH:
Aminoglycosides;
Antioxidants*;
Cell Death;
Cell Line;
Electrocardiography;
Gentamicins;
Oxidative Stress;
Reactive Oxygen Species;
Streptomycin;
Taurine;
Tea;
Vitamin E;
Vitamins
- From:Korean Journal of Aerospace and Environmental Medicine
2005;15(1):18-22
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Aminoglycosides that are widely used in the treatment of Gram (-)-infected diseases have side effects such as ototoxicity and nephrotoxicity. These side effects are closely related to oxidative stress. The purpose of this study was to evaluate the effect of antioxidants on the aminoglycoside-induced cochlear cell damage. METHODS: HEI-OC1 was used as an experimental cell line. The number of cells was counted after administration of streptomycin or gentamicin plus Vitamin E, taurine, or one of the components of green tea (EGCG, EGC, ECG, EC) under cell proliferating conditions (33oC). RESULTS: Streptomycin and gentamicin decreased the number of HEI-OC1 cells in a dose dependent manner. Aminoglycoside-induced cell death was recovered by the addition of Vitamin E, taurine, or green tea. CONCLUSION: These results suggest that Vitamin E, taurine, or green tea components block aminoglycoside-induced ototoxicity via the suppression of the increased production of reactive oxygen species.
