The Effects of Venlafaxine on Neurite Growth of PC12 Cells.
- Author:
Hong Seok OH
1
;
Joon Ho CHOI
;
Jun Seok LEE
;
Joon Noh LEE
;
Mi Ran CHOI
;
Young Gyu CHAI
;
Seok Hyeon KIM
;
Byung Hwan YANG
Author Information
1. Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Antidepressant;
PC12 cell;
Neurite growth;
NGF
- MeSH:
Animals;
Antidepressive Agents;
Nerve Growth Factor;
Neurites*;
Neurons;
PC12 Cells*;
Venlafaxine Hydrochloride
- From:Journal of the Korean Society of Biological Psychiatry
2003;10(2):126-132
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The purpose of this study is to examine the effects of venlafaxine, one of novel antidepressant drugs, on neurite growth in PC12 cells. METHODS: PC12 cells were cultured with NGF for eight days. Then different concentrations(0micrometer, 1micrometer, 5micrometer) of venlafaxine were mixed with cultured PC12 cells. After 24 hours and 48 hours of culture, we compared the effects of venlafaxine on the total length of neurites of cultured PC12 cells between no venlafaxine treated group(0micrometer) and venlafaxine treated groups(1micrometer and 5micrometer). Additionally, we studied the concentration-dependent effect of venlafaxine on differentiation in PC12 cells. RESULTS: Experimental results showed that 1) the mean length of neurites in 1micrometer and 5micrometer venlafaxine treated group was more increased than no venlafaxine treated group(p=0.002). 2) the length of neurite in 5micrometer venlafaxine treated group was more elongated than 1micrometer venlafaxine treated group(p=0.046). 3) the length of neurite in 6micrometer venlafaxine treated group was more elongated than all the other concentrations in our experiment. Above 6micrometer, the length of neurite was shortened in inverse proportion to the concentration of venlafaxine. CONCLUSIONS: This results suggest that venlafaxine, one of novel antidepressant drugs, promotes the differentiation of neuron. This study is believed to be a first step toward understanding the molecular and cellular mechanisms of antidepressant treatment.
