Effect of Hypoxia on the Melanogenesis of Murine B16 Melanoma Cells.
- Author:
Young Gil SONG
1
;
Jeong Yeh YANG
;
Jeung Hyun KOO
;
Ha Yong YOON
;
Seung Yong LEE
;
Seok Kweon YUN
;
Byung Hyun PARK
;
Han Uk KIM
;
Jin Woo PARK
Author Information
1. Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Korea. jinwoo@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
cAMP;
Hypoxia;
Melanogenesis;
Tyrosinase
- MeSH:
Anoxia*;
Blotting, Western;
Melanins;
Melanoma;
Melanoma, Experimental*;
Monophenol Monooxygenase;
Oxygen;
RNA, Messenger
- From:Korean Journal of Dermatology
2007;45(4):332-337
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Numerous reports suggest the role of oxygen in melanogenesis. However, little has been reported on the effect of a hypoxic environment on cellular melanogenesis. OBJECTIVE: The effect of low oxygen tension on cellular melanogenesis was investigated in B16 murine melanoma cells. METHODS: Using cells cultured under an ambient (21% O2) or hypoxic (5% O2) condition, melanin content and tyrosinase activity were measured spectrophotometrically. The expression of tyrosinase, tyrosinase-related protein (TRP)- 1, and TRP-2 were analyzed by RT-PCR and Western blot. RESULTS: Culture of cells under hypoxic conditions caused significant inhibition of isobutylmethylxanthine (IBMX)- induced increase of melanin content. No cytotoxicity was observed during the hypoxic culture periods. Decreased melanin content occurred through the decrease of tyrosinase protein and activity (p<0.01). The mRNA levels of tyrosinase and TRP-2 were also decreased by hypoxia, while that of TRP-1 was unchanged. Similar inhibitions of melanin content and tyrosinase activity were observed in the cells stimulated with dibutyryl-cAMP. CONCLUSION: IBMX-induced melanogenesis in B16 cells was significantly inhibited under hypoxic culture conditions, suggesting the important role of oxygen tension in cellular melanogenesis.
