Expression of Caveolin-3 in the Muscle Cell and Tissue.
- Author:
Bum Sun KWON
1
;
Seong Jae LEE
;
Jung Keun HYUN
;
Dong Jin JUN
;
Hyung Wook JOO
;
Byung Hee KIM
;
Dong Hoon SHIN
Author Information
1. Department of Rehabilitation Medicine, Dankook University College of Medicine, Korea. bskwon@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Caveolin-3;
Dystrophin;
Duchenne muscular dystrophy;
Myoblast;
Skeletal muscle
- MeSH:
Animals;
Organelle Biogenesis;
Blotting, Western;
Caveolae;
Caveolin 3*;
Cell Membrane;
Dystrophin;
Humans;
Immunohistochemistry;
Membranes;
Muscle Cells*;
Muscle, Skeletal;
Muscular Dystrophy, Duchenne;
Myoblasts;
Myoblasts, Skeletal;
Rats;
Regeneration;
Signal Transduction
- From:Journal of the Korean Academy of Rehabilitation Medicine
2003;27(3):382-387
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Caveolae are the microdomain of the plasma membrane that have been implicated in signal transduction and caveolin is a principal component of the caveolae. Caveolin-3, a family of caveolin related protein, is expressed only in muscle tissue. Here we examined the expression of caveolin-3 in the course of myobalst differentiation and within the muscle tissue. METHOD: L6 cell, rat skeletal myoblast, was cultured in the low mitogen medium and caveolin-3 expression was observed both by immunocytochemistry and western blot analysis. Localization of caveolin-3 within the muscle tissue was investigated and compared to that of dystrophin. RESULTS: While caveolin-3 was not expressed in the proliferating myolast, caveolin-3 was expressed in the differentiated myoblast. Caveolin-3 and dystrophin were co-expressed in the membrane of muscle tissue and integrated density of caveolin-3 was elevated in the area of muscle injury. In the Duchenne muscular dystrophy, caveolin-3 was expressed in the membrane of muscle tissue, but dystrophin was not. CONCLUSION: Caveolin-3 was induced during the myobalst differentiation and its expression was increased during the muscle regeneration. Caveolin-3 was physically associated with dystrophin as a complex, but not absolutely required for the biogenesis of dystrophin complex.
