Neuroprotective Effect of Exogenous Growth Hormone in Brain Injury-Rat Model.
- Author:
Hee Seung YANG
1
;
Min Ho CHUN
;
Don Kyu KIM
;
Sang Tae KIM
;
Hee Jin JO
Author Information
1. Department of Rehabilitation Medicine, Soonchunhyang University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Growth hormone;
Traumatic brain injury;
Motor function test;
Maze test
- MeSH:
Animals;
Brain Injuries;
Brain*;
Cell Death;
Growth Hormone*;
In Situ Nick-End Labeling;
Neurons;
Neuroprotective Agents*;
Percussion;
Rats;
Rats, Sprague-Dawley;
Tolonium Chloride
- From:Journal of the Korean Academy of Rehabilitation Medicine
2006;30(4):333-339
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the neuroprotective effect of growth hormone (GH) following lateral fluid percussion brain injury. METHOD: Sprague-Dawley rats weighing 200~250 g were subjected to moderate lateral fluid percussion brain injury and evaluated at 1, 7, 14 and 28 days post-injury. Rats were divided into control and GH pretreatment groups. GH (10 microgram/10microliter) or normal saline (10microgram/10microliter) was administered by intracerebro-ventricular injection 30 min before brain injury. We measured histopathological changes with TUNEL and toluidine blue Nissl stain and conducted motor function tests and an 8-arm radial maze test to identify neurobehavioral deficits and cognitive deficits, respectively, at one day to 4 weeks post-injury. RESULTS: In the pretreated GH group, a significant reduction in TUNEL-positive cells was found compared with the control group at 1 day and 1 week post-injury (p<0.05). The pretreated GH group showed significantly greater improvement than controls motor function tests at 1 day post-injury (p<0.05) and in an 8-arm radial maze test at 2 and 4 weeks post-injury (p<0.05). CONCLUSION: These findings suggested that GH had a neuroprotective effect in neuronal cell death, motor function and cognitive deficits following experimental brain injury.
