Knockdown of 14-3-3zeta enhances radiosensitivity and radio-induced apoptosis in CD133+ liver cancer stem cells.
- Author:
Young Ki LEE
1
;
Wonhee HUR
;
Sung Won LEE
;
Sung Woo HONG
;
Sung Woo KIM
;
Jung Eun CHOI
;
Seung Kew YOON
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: 14-3-3zeta; apoptosis; cancer stem-like cell; hepatocellular carcinoma; radioresistance
- MeSH: 14-3-3 Proteins/genetics/*metabolism; Antigens, CD/genetics/*metabolism; Apoptosis Regulatory Proteins/genetics/metabolism; Carcinoma, Hepatocellular/genetics/metabolism; Cell Line, Tumor; *Gamma Rays; Glycoproteins/genetics/*metabolism; Humans; Liver Neoplasms/genetics/metabolism; Neoplastic Stem Cells/metabolism/*radiation effects; Peptides/genetics/*metabolism; *Radiation Tolerance
- From:Experimental & Molecular Medicine 2014;46(2):e77-
- CountryRepublic of Korea
- Language:English
- Abstract: 14-3-3zeta is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3zeta decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3zeta affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3zeta decreased cell viability and the number of spheres by reducing radioresistance in CSCs after gamma-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3zeta after IR. These results suggest that 14-3-3zeta knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs.
