Myogenic Differentiation of Human Adipose-Derived Stem Cells.
- Author:
Yoon Ghil PARK
1
;
Ah Mi BAEK
;
Byung Rok DO
;
Jung Hwa CHOI
;
Sun Do KIM
Author Information
1. Department of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 135-720, Korea.
- Publication Type:Original Article
- Keywords:
Muscular dystrophy;
Stem cell;
Differentiation;
Myogenic regulatory factor;
Desmin
- MeSH:
Adipose Tissue;
Complement System Proteins;
Culture Media;
Desmin;
Genes, vif;
Humans;
Lipectomy;
Muscular Dystrophies;
Muscular Dystrophy, Duchenne;
Myoblasts;
Myogenic Regulatory Factors;
Phenotype;
Proteins;
RNA, Messenger;
Stem Cells;
Tissue Therapy
- From:Journal of the Korean Academy of Rehabilitation Medicine
2011;35(1):8-13
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Cell therapy has been extensively studied as a gene complementation approach in muscular dystrophy including Duchenne muscular dystrophy (DMD), and adipose tissue has recently been identified as a uniquely abundant and adequately accessible source of pluripotent cells. In the present work, we investigated myogenic potentials of adipose-derived stem cells (ADSCs) depending on culture media and isolation with using surface markers. METHOD: Human ADSCs were obtained by liposuction and cultured in two different media; control and myogenic media. In addition we attempted to isolate ADSCs by utilizing surface markers: CD45 and CD133. The following observations were made to evaluate myogenic differentiation as the expression of myogenic regulatory factors (MyoD, Myf-5 and Myf-6) and desmin by RT-PCR and immunoflurescence study. RESULTS: Conversion of ADSCs to myogenic phenotype was observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type and isolation method. In addition mRNA of MyoD and Myf-5 were positive in both culture media, and there were no differences of MyoD and Myf-5 responses between CD45- and CD45-CD133-ADSCs. However, secondary myogenic regulatory factor (Myf-6) was not expressed constantly, and desmin were negative in all cultural condition. CONCLUSION: Our findings suggest that human ADSCs might have myogenic potentials. However, further studies are needed to express the secondary myogenic regulatory factors and proteins in myoblasts.
