Effect of Conditioned Ischemic Stresses on NF-kappaB Activation in Rat Skeletal Muscle.
- Author:
Jeong Eun LEE
1
;
Chyung Ki LEE
;
Tae Sik YOON
;
Jong Sik HAH
Author Information
1. Department of Rehabilitation Medicine, Ewha Womans University College of Medicine, Korea. ipray@hosanna.net
- Publication Type:Original Article
- Keywords:
NF-kappa;
Ischemic stress;
Electrical stimulation;
Genistein;
Skeletal muscle
- MeSH:
Animals;
Constriction;
Dimethyl Sulfoxide;
Edema;
Electric Stimulation;
Electrophoretic Mobility Shift Assay;
Femoral Nerve;
Genistein;
Ischemia;
Muscle Contraction;
Muscle, Skeletal*;
Muscles;
NF-kappa B*;
Quadriceps Muscle;
Rats*;
Reperfusion
- From:Journal of the Korean Academy of Rehabilitation Medicine
2003;27(6):990-996
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the effect of conditioned ischemic stresses on NF-kappaB activities and the protective role of genistein in rat skeletal muscle. METHOD: Rectus femoris muscles of rats were divided into 4 groups according to conditioned ischemic stresses as followings: sham-operated control group; 2 hour ischemia group by clamping the proximal femoral vessel; ischemia-reperfusion group by 30 minutes of reperfusion following 2 hours of ischemia; ischemia-reperfusion-electrical nerve stimulation group in which current was applied at the femoral nerve during 30 min of reperfusion period to induce repetitive muscle contraction. Either genistein-5% dimethyl sulfoxide (DMSO) or DMSO was injected 2 hours before ischemic induction. NF-kappaB activities were analysed by electrophoretic mobility shift assay. RESULTS: NF-kappaB activities pretreated with DMSO were significantly increased in ischemia, ischemia-reperfusion and ischemia-reperfusion-electrical nerve stimulation group compared with those of control group and in ischemia-reperfusion-electrical nerve stimulation group compared with those of ischemia group. Muscle edema was increased in ischemia-reperfusion-electrical nerve stimulation group. But, NF-kappa activities and muscle edema pretreated with genistein were attenuated in ischemia-reperfusion-electrical nerve stimulation group compared with those pretreated with DMSO alone. CONCLUSION: NF-kappaB activities reflect the severity of ischemic stresses. Pretreatment with genistein can attenuate NF-kappa activities and muscle edema in ischemic stress of rat skeletal muscle.
