Expression of Growth Factors and Cytokines in Patients withDegenerated Disc Disease and Herniated Nucleus Pulposus.
- Author:
Chan Sam MOON
1
;
Seung Cheol LEE
;
Woon Won JUNG
;
Donggeun SUL
;
Young Ki HONG
;
Hyun Sook KIM
;
Joonho WANG
;
Sang Heon LEE
Author Information
1. Department of Orthopaedic Surgery, Sung-Ae Hospital, Korea. spinelee@gmail.com
- Publication Type:Original Article
- Keywords:
Herniated nucleus pulposus;
Degenerated disc disease;
Cytokines;
Growth factors
- MeSH:
Back Pain;
Blotting, Western;
Cytokines;
Dichlorodiphenyldichloroethane;
Humans;
Intercellular Signaling Peptides and Proteins;
Interleukin-6;
Interleukin-8;
Intervertebral Disc;
Intervertebral Disc Degeneration;
Intervertebral Disc Displacement;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Academy of Rehabilitation Medicine
2009;33(3):304-308
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the expression of cytokines and growth factors in disc specimens obtained from small numbers of patients with herniated nucleus pulposus (HNP) and degenerated disc disease (DDD). METHOD: A total of ten human intervertebral disc samples consisted of five painful degenerative discs and five herniated intervertebal discs were obtained from surgery. MRI and Western blot analyses on these disc samples were performed to determine the levels of disc degeneration and the expression levels of cytokines and growth factors. RESULTS: The levels of IL-6 were significantly greater in the DDD patients than in the HNP patients, but no statistical differences were observed in the expression of IL-1beta, IL-8 and TNF-alpha between the HNP and DDD patients. In addition, the expression of TGF beta was significantly higher in the DDD patients than in the HNP patients. CONCLUSION: The higher levels of cytokine and growth factor expression in the DDD than in the HNP show why discogenic patients usually have more severe back pain than patients with herniated discs.
