Effects on Myoblast Proliferation by Fibroblast Growth Factor, Leukemia Inhibitory Factor and Interleukin-1.
- Author:
Yoon Ghil PARK
1
Author Information
1. Department of Rehabilitation Medicine, Yonsei University College of Medicine, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Myoblast;
Growth factor;
Basic fibroblast growth factor (bFGF);
Leukemia inhibitory factor (LIF);
Interleukin- 1 (IL-1)
- MeSH:
Biopsy;
Complement System Proteins;
Fibroblast Growth Factor 2;
Fibroblast Growth Factors*;
Fibroblasts*;
Genes, vif;
Humans;
Intercellular Signaling Peptides and Proteins;
Interleukin-1*;
Leukemia Inhibitory Factor*;
Leukemia*;
Muscle Fibers, Skeletal;
Muscular Dystrophy, Duchenne;
Myoblasts*
- From:Journal of the Korean Academy of Rehabilitation Medicine
2002;26(4):426-431
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Recently, cultured myoblast transplantation has been extensively studied as a gene complementation approach in such genetic diseases as Duchenne muscular dystrophy (DMD). In the present work we investigated the stimulating effects of the growth factors, such as basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF) and interleukin-1 (IL-1), on growth rate and differentiation of myoblast. METHOD: Human myoblasts were cultured from biopsy and treated in vitro with various concentration of bFGF, LIF and IL-1. In serum-free defined medium the following observation were made to evaluate differentiation. RESULTS: bFGF and LIF except IL-1 were found to have stimulating effect of myoblast proliferation comparing to control group (p<0.05), yet there were no statistically significant differences among each growth factors (p<0.05). The most significant growth stimulation of myoblasts in culture was achieved by adding 3.0 ng/ml of bFGF, producing a stimulation effect up to 2.01-fold. All myoblasts treated with growth factors differentiated into myotube. CONCLUSION: Our findings indicate that bFGF and LIF stimulate the proliferation of myoblast, which may result in an effective way in producing large numbers of myoblasts for clinical myoblast transplantation in DMD patients.
