The Effect of Steroid on Heat Shock Protein 70 Expression in mdx Mice.
- Author:
Jeong Hoon LIM
1
;
Moon Suk BANG
Author Information
1. Department of Rehabilitation Medicine, Konkuk University Medical Center, Korea. jhlim@kuh.ac.kr
- Publication Type:Original Article
- Keywords:
mdx mouse;
Steroid;
Exercise;
Heat shock protein
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Heat-Shock Proteins;
Hot Temperature;
HSP70 Heat-Shock Proteins;
Mice;
Mice, Inbred mdx;
Models, Animal;
Muscular Dystrophies
- From:Journal of the Korean Academy of Rehabilitation Medicine
2009;33(1):1-4
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the effect of steroid administration on the apoptosis and heat shock protein 70 (HSP70) expression after exercise in the animal model of Duchenne muscular dystrophy. METHOD: We measured Bcl-2, BAX and HSP70 expression by western blotting. 20 control and 20 mdx mice were divided into free-living (n=10) and exercise (n=10) groups. Free-living and exercise groups were further divided into steroid-treated and sham-treated groups to evaluate the effect of steroid administration. RESULTS: Apoptosis was most prominent in the sham-treated exercise group, while apoptosis was significantly reduced in the steroid-treated exercise group. HSP70 expression was maximized in sham-treated exercise group, whereas steroid administration inhibited HSP70 expression after exercise in muscular dystrophy animal model. Exercise loading was found to cause severe apoptosis but steroid administration alleviated apoptotic damage in mdx mice. CONCLUSION: HSP70 expression was suppressed in the steroid-treated exercise group, which suggests steroid might have major preventive effect in exercise-induced apoptosis of muscular dystrophy animal model.
