Immunoreactivity of Iron-Repressible Cell Wall Proteins and Exoproteins of Staphylococcus aureus.
- Author:
Yong LIM
1
;
Jong Hoon PARK
;
Seung IL LEE
;
Soo Young CHO
;
Sung Heui SHIN
Author Information
1. Department of Microbiology, Chosun University Medical School, Kwangju, Korea. shsin@chosun.ac.kr
- Publication Type:Note
- MeSH:
Blotting, Western;
Cell Wall*;
Electrophoresis, Polyacrylamide Gel;
Humans;
Sepsis;
Staphylococcus aureus*;
Staphylococcus*;
Virulence
- From:
Infection and Chemotherapy
2004;36(4):241-244
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, we tried to investigate immunoreactivity of iron-repressible cell wall proteins and exoproteins of Staphylococcus aureus when the convalescent serum obtained from the patient with septicemia was applied. On SDS-PAGE and western blot analysis, several iron-repressible cell wall proteins expressed in iron-deficient BHI showed strong immunoreactivity, whereas no or relatively weak immunoreactivity was shown in iron-sufficient BHI. Several exoproteins were expressed only in iron-deficient BHI and these exoproteins showed strong immunoreactivity. These results indicate that several iron-repressible cell wall proteins and exoproteins are expressed and are immunogenic in vivo. Since in vivo state is an relatively iron-restricted condition, we recommend the use of iron-deficient media for studies concerning pathogenicity of staphylococcal in human.
