Genotypic Diversity of Multidrug Resistant Shigella species from Iran.
- Author:
Sajjad ZAMANLOU
1
;
Mohammad Ahangarzadeh REZAEE
;
Mohammad AGHAZADEH
;
Reza GHOTASLOU
;
Hossein Hosseini NAVE
;
Younes KHALILI
Author Information
- Publication Type:Original Article
- Keywords: Shigella; Integron; Multidrug resistant; Enterobacterial repetitive intergenic consensus; Polymerase chain reaction
- MeSH: Ampicillin; Clone Cells; Consensus; Developing Countries; Diffusion; Dysentery, Bacillary; Genotype; Integrons; Iran*; Methods; Phenotype; Polymerase Chain Reaction; Prevalence; Shigella*; Streptomycin; Tetracycline; Trimethoprim, Sulfamethoxazole Drug Combination
- From:Infection and Chemotherapy 2018;50(1):29-37
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: In many developing countries, shigellosis is endemic and also occurs in epidemics and treatment of multidrug-resistant (MDR) isolates are important. The aims of this study were to determine the antimicrobial susceptibility, prevalence of class 1 and 2 integrons and the clonal relatedness of isolates. MATERIALS AND METHODS: Antimicrobial susceptibility tests were performed by disc diffusion method. Polymerase chain reaction (PCR)-sequencing technique was employed for detection and characterization of integrons. The genetic relatedness was evaluated by using enterobacterial repetitive intergenic consensus (ERIC) PCR. RESULTS: There was a high percentage of resistance to trimethoprim-sulfamethoxazole (TMP/SMX) (93.7%), ampicillin (AMP) (87.3%), streptomycin (STR) (84.5%) and tetracycline (TET) (78.9%). Multidrug resistant phenotype was seen in 95.1% of total isolates. Most common MDR profile was TMP/SMX/STR/AMP resistant pattern. Among the 142 Shigella spp. analyzed in this study, 28 isolates were positive for class 1 integron with two types of gene cassette arrays (dfrA17/aadA5 = 31.7% and dfrA7 = 3.8%). The class 2 integron was more frequently detected among the isolates (94.7%) with dfrA1/sat1/aadA1 (69.4%) and dfrA1/sat1 (30.6%) gene cassettes. ERIC-PCR results showed 6, 5, 4 and 3 main genotypes among S. flexneri, S. sonnei, S. boydii and S. dysenteriae isolates, respectively. CONCLUSIONS: Our findings revealed that multidrug resistant Shigella species with high prevalence of class 2 integron were very common in Iran. In addition, ERIC-PCR patterns showed limited variety of clones are responsible for shigellosis in the region of the study.
