Evaluation of a New Diagnostic Test for Invasive Fungal Infections Using Prophenoloxydase System to Measure (1->3)-beta-D-glucan Concentration.
- Author:
Ki Deok LEE
1
;
Jong Won YOON
;
Jung Shik BAE
;
Joong Hyuck AUH
;
Bok Luel LEE
;
Eu Suk KIM
;
Wan Beom PARK
;
Hong Bin KIM
;
Nam Joong KIM
;
Myoung Don OH
;
Kang Won CHOE
Author Information
1. Department of Internal Medicine, Eulji University School of Medicine, Daejeon, Korea.
- Publication Type:Original Article
- Keywords:
Invasive fungal infection;
Non-invasive diagnosis;
(1-->3)-beta-D-glucan;
Prophenoloxydase system
- MeSH:
Diagnostic Tests, Routine*;
Early Diagnosis;
Healthy Volunteers;
Humans;
Plasma;
Prognosis;
Sensitivity and Specificity
- From:
Infection and Chemotherapy
2006;38(3):146-153
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Prognosis of invasive fungal infection is supposed to be better when diagnosed earlier. (1->3)-beta-D-glucan (BDG) test is considered useful in early diagnosis of invasive fungal infections among high-risk patients. A new diagnostic test using prophenoloxydase system to measure BDG level is developed. A study was performed to evaluate clinical usefulness of this new diagnostic test. MATERIALS AND METHODS: 15 patients of confirmed invasive fungal infections and 38 healthy normal volunteers were enrolled. Plasma or serum BDG concentrations were measured using prophenoloxydase system. Assays for intra-run variability and inter-run variability were performed. A cut-off value was determined and sensitivity and specificity of the test were evaluated. RESULTS: A cut-off value of 94.90 pg/mL was determined. Sensitivity and specificity of the test were 86.7% and 52.6%, respectively. Statistical analyses of inter-run variability and intra-run variability revealed the test is reliable (P< or =0.001). CONCLUSION: BDG test using prophenoloxydase system is a sensitive and reliable test in non-invasive detection of invasive fungal infection.
